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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Huntingtin Polyclonal antibody (27206-1-AP)

    IHC staining of human cerebellum using 27206-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Huntingtin Polyclonal antibody (27206-1-AP)

    IHC staining of human cerebellum using 27206-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Huntingtin Polyclonal antibody (27206-1-AP)

    IHC staining of human brain using 27206-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Huntingtin Polyclonal antibody (27206-1-AP)

    IHC staining of human brain using 27206-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of rat cerebellum tissue using Huntingtin Polyclonal antibody (27206-1-AP)

    IF Staining of rat cerebellum using 27206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Huntingtin Polyclonal antibody (27206-1-AP)

    IF Staining of mouse brain using 27206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 27206-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

HTT, HD, HD protein, Huntingtin, myristoylated N-terminal fragment, Huntington disease protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat cerebellum tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27206-1-AP targets Huntingtin in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25922

Product name: Recombinant human Huntingtin protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 2845-3000 aa of NM_002111

Sequence: LDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGE

相同性解析による交差性が予測される生物種
Full Name huntingtin
Calculated molecular weight 348 kDa
GenBank accession numberNM_002111
Gene Symbol Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2880799
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP42858
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HTT(huntingtin), also named HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington's disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders, and dementia.

Protocols

Product Specific Protocols
IF protocol for Huntingtin antibody 27206-1-APDownload protocol
IHC protocol for Huntingtin antibody 27206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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