Human uPAR, PLAUR ELISA Kit0 Publications

カタログ番号: KE00206

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human uPAR, PLAUR ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants and Urine

アッセイ方法:
Sandwich

感度:
7.0 pg/mL

測定範囲:
62.5-4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 89% 76%-112%
Cell culture supernatants 105% 82%-119%
Urine 90% 77%-111%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 256.3 12.5 4.9
2 20 512.2 16.3 3.2
3 20 2,009.1 49.8 2.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 259.7 21.6 8.3
2 24 501.1 28.5 5.7
3 24 1,863.5 154.6 8.3

製品概要
KE00206 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The uPAR ELISA kit is to be used to detect and quantify protein levels of endogenous uPAR. The assay recognizes human uPAR. An antibody specific for uPAR has been pre-coated onto the microwells. The uPAR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for uPAR is added to detect the captured uPAR protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD87, MO3, PLAUR, U PAR, UPAR, URKR
背景説明

uPAR is a highly glycosylated GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. suPAR has been detected in plasma, and increased plasma concentrations of suPAR have been found in patients with some advanced cancers.


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