Speedy™ Human uPAR/CD87 One-Step ELISA Kit
Sensitivity
14.0 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : SE50053
Synonyms
PLAUR, CD87, MO3, U PAR, UPAR
Validation Data Gallery
Product Information
| Product name | Speedy™ Human uPAR/CD87 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 14.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5329 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 94%-103% |
| Cell culture supernatant | 109% | 106%-113% |
| Urine | 106% | 101%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,050.7 | 39.8 | 1.9 |
| 2 | 8 | 507.2 | 12.3 | 2.4 |
| 3 | 8 | 155.4 | 4.1 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,058.5 | 38.2 | 1.9 |
| 2 | 16 | 506.4 | 9.2 | 1.8 |
| 3 | 16 | 151.0 | 6.4 | 4.2 |
Background Information
uPAR/PLAUR is a highly glycosylated GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. suPAR has been detected in plasma, and increased plasma concentrations of suPAR have been found in patients with some advanced cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PLAUR, CD87, MO3, U PAR, UPAR |