Recombinant Human A2ML1 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4523
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human A2ML1 protein Glu18-Glu1454 (Accession# A8K2U0) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 144568 |
| Accession | A8K2U0 |
| PredictedSize | 186.5 kDa |
| SDS-PAGE | 150-250 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
A2ML1 is a monomeric protease inhibitor belonging to the A2M superfamily of protease inhibitors and complement factors. A2ML1 is abundant in the epidermal granular layer of the skin but is also found in the placenta, thymus, and testis. Similar to the functional characteristics of A2Ms, the A2ML1 protein can inhibit extracellular protease through a unique “capture” mechanism, thereby contributing significantly to epithelial cell desquamation. Moreover, A2ML1 can regulate epithelial cell homeostasis by interacting with growth factors and cytokines.
References:
1.Nielsen, Nadia Sukusu et al. Nature communications vol. 13,1 (2022): 3033. 2.Zhang, Xiaoyun et al. Canadian journal of gastroenterology & hepatology vol. 2023 (2023): 5557546.
