Human ZEB1 ELISA Kit
Sensitivity
1.0 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00942
Synonyms
AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8
Validation Data Gallery
Product Information
KE00942 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ZEB1 ELISA kit is to be used to detect and quantify protein levels of endogenous human ZEB1. The assay recognizes human ZEB1. An antibody specific for human ZEB1 has been pre-coated onto the microwells. The human ZEB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ZEB1 is added to detect the captured human ZEB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ZEB1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6935 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 108% | 101%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,304.8 | 52.7 | 4.0 |
| 2 | 8 | 287.9 | 8.8 | 3.0 |
| 3 | 8 | 165.3 | 11.8 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,252.9 | 88.6 | 7.1 |
| 2 | 16 | 288.7 | 13.8 | 4.8 |
| 3 | 16 | 155.7 | 14.6 | 9.4 |
Background Information
Transcription factor zinc finger E-box binding homeobox 1 (ZEB1) is a transcription factor critical in epithelial-to-mesenchymal transition (EMT), cancer progression, and metastasis. It can promote not only the movement and diffusion of tumor cells but also cell stemness, treatment resistance, tumor metastasis and immune escape. The expression of ZEB1 is strictly regulated by a variety of pre-transcriptional and post-transcriptional signaling pathways and molecules. Increasing evidence indicates that protein modifications such as methylation and acetylation of ZEB1 can also affect tumor metastasis. More importantly, ZEB1 induces immunosuppressive cells and chemokines into the tumor microenvironment (TME), leading to the formation of a tumor immunosuppressive microenvironment.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8 |