Human YTHDC2 ELISA Kit
Sensitivity
0.05 ng/mL
Range
0.25-16 ng/mL
Reactivity
Human
Cat no : KE00715
Synonyms
3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2
Validation Data Gallery
Product Information
KE00715 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human YTHDC2 ELISA kit is to be used to detect and quantify protein levels of endogenous human YTHDC2. The assay recognizes human YTHDC2. An antibody specific for human YTHDC2 has been pre-coated onto the microwells. The human YTHDC2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human YTHDC2 is added to detect the captured human YTHDC2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human YTHDC2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 64848 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 88%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.23 | 0.76 | 8.20 |
| 2 | 8 | 2.18 | 0.14 | 6.28 |
| 3 | 8 | 1.24 | 0.12 | 9.73 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.43 | 1.10 | 11.68 |
| 2 | 16 | 2.21 | 0.16 | 7.19 |
| 3 | 16 | 1.27 | 0.10 | 7.72 |
Background Information
YTH domain-containing 2 (YTHDC2) is a member of the YTH family of proteins, which has been shown to regulate a variety of mRNA metabolisms, including splicing, export, degradation, and translation. It has been reported that YTHDC2 plays important roles in various physiological and pathological processes. For instance, YTHDC2 is deeply involved in germ cell differentiation. In tumorigenesis, YTHDC2 is also required for the translation of proteins that induce malignant phenotypes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2 |