Speedy™ Human VWF One-Step ELISA Kit
Sensitivity
0.05 ng/mL
Range
0.47-30 ng/mL
Reactivity
Human
Cat no : SE50036
Synonyms
VWF, VWFpp, F8VWF, von Willebrand antigen II, VWD
Validation Data Gallery
Product Information
| Product name | Speedy™ Human VWF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7450 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 103% | 99%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.24 | 0.24 | 1.57 |
| 2 | 8 | 3.65 | 0.05 | 1.37 |
| 3 | 8 | 1.86 | 0.03 | 1.61 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.24 | 0.27 | 1.77 |
| 2 | 16 | 3.72 | 0.08 | 2.15 |
| 3 | 16 | 1.94 | 0.09 | 4.64 |
Background Information
Von Willebrand factor (VWF), the largest circulatory glycoprotein, supports shear stress-associated platelet adhesion and aggregation at sites of vascular injury. It is an array of multimeric (concatameric), disulfide‐linked, mirror‐symmetric glycoprotein dimers called protomers. The mature VWF monomer is a 2050‐residue protein (MW ~ 250 kDa) containing 12 N‐linked and 10 O‐linked oligosaccharide chains.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | VWF, VWFpp, F8VWF, von Willebrand antigen II, VWD |