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Recombinant Human VEGF-B protein (hFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

hFc Tag

Activity

not tested

Cat no : Eg0123

Validation Data Gallery

  • Purity of Recombinant Human VEGF-B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human VEGF-B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • The purity of Human VEGF-B was greater than 90% as determined by SEC-HPLC.

    The purity of Human VEGF-B was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human VEGF-B protein Pro22-Ala207 (Accession# P49765-1) with a human IgG1 Fc tag at the N-terminus.
GeneID 7423
Accession P49765-1
PredictedSize 45.4 kDa
SDS-PAGE 40 kDa and 50-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Vascular endothelial growth factor B (VEGFB) is a member of the PDGF/VEGF growth factor family. It is produced as a secreted homodimer. VEGF-B binds to VEGF receptor-1 (Flt-1) and regulates plasminogen activator activity in endothelial cells. It also binds to Neuropilin-1 (NRP1). There are two splice isoforms, VEGF-B (167) and VEGF-B (186), which have identical NH(2)-terminal cystine knot growth factor domains but differ in their COOH-terminal domains. VEGF-B (167) has a heparin-binding domain so can bind to cell-surface heparan sulphate proteoglycans upon secretion, while VEGF-B (186) does not contain the heparin-binding domain.

References:

1. B Olofsson, et al. (1998) Proc Natl Acad Sci U S A. 95(20):11709-14. 
2. T Makinen, et al. (1999) J Biol Chem. 274(30):21217-22. 
3. Xuri Li. (2010) Cell Res. 20(7):741-4.
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