Speedy™ Human Tie2 One-Step ELISA Kit
Sensitivity
0.06 ng/mL
Range
0.313 -20 ng/mL
Reactivity
Human
Cat no : SE50235
Synonyms
TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Tie2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.313 -20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7010 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 110% | 106%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.28 | 0.30 | 3.23 |
| 2 | 8 | 2.34 | 0.07 | 2.99 |
| 3 | 8 | 1.32 | 0.02 | 1.52 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.49 | 0.43 | 4.53 |
| 2 | 16 | 2.33 | 0.06 | 2.58 |
| 3 | 16 | 1.33 | 0.03 | 2.26 |
Background Information
Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. Tie2 acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene of Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Tie2 can also exist as a soluble form (sTie2) which is released from endothelial cells and present in human blood. The concentration of sTie2 is increased in a range of diseases, including peripheral arterial disease and myocardial infarction.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1 |