Speedy™ Human TSLP One-Step ELISA Kit
Sensitivity
0.7 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : SE50182
Synonyms
Thymic stromal lymphopoietin
Validation Data Gallery
Product Information
| Product name | Speedy™ Human TSLP One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 85480 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 80% | 73%-90% |
| Cell culture supernatant | 99% | 94%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 290.8 | 13.3 | 4.6 |
| 2 | 8 | 66.2 | 3.5 | 5.3 |
| 3 | 8 | 32.6 | 0.9 | 2.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 285.5 | 16.6 | 5.8 |
| 2 | 16 | 65.6 | 4.1 | 6.3 |
| 3 | 16 | 32.4 | 1.1 | 3.4 |
Background Information
Thymic stromal lymphopoietin (TSLP) is a multifunctional cytokine primarily produced by epithelial cells in response to tissue damage, infection, or allergen exposure. It plays a significant role in immune regulation, inflammation, and cancer. TSLP acts through its receptor complex, composed of TSLP receptor (TSLPR) and interleukin-7 receptor alpha chain (IL-7Rα), to activate downstream signaling pathways, including JAK-STAT, PI3K-AKT, and MAPK. TSLP is involved in various physiological and pathological conditions, including asthma, allergic diseases, and chronic inflammatory diseases. It promotes type 2 immune responses by inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c(+) dendritic cells. Additionally, TSLP plays a role in tissue homeostasis, host defense, and microbiome regulation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Thymic stromal lymphopoietin |