Human TRPS1 ELISA Kit
Sensitivity
5.5 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00568
Synonyms
TRPS1, GC79, LGCR, Tricho-rhino-phalangeal syndrome type I protein
Validation Data Gallery
Product Information
KE00568 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TRPS1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TRPS1. The assay recognizes human TRPS1. An antibody specific for human TRPS1 has been pre-coated onto the microwells. The human TRPS1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TRPS1 is added to detect the captured human TRPS1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TRPS1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.5 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7227 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 97%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,331.6 | 61.8 | 4.6 |
| 2 | 8 | 329.0 | 14.6 | 4.4 |
| 3 | 8 | 166.4 | 9.2 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,379.0 | 76.5 | 5.5 |
| 2 | 16 | 342.6 | 17.5 | 5.1 |
| 3 | 16 | 175.2 | 11.6 | 6.6 |
Background Information
TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA, with nine zinc finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TRPS1, GC79, LGCR, Tricho-rhino-phalangeal syndrome type I protein |