Human TP53BP1 ELISA Kit
Sensitivity
0.06 ng/mL
Range
0.94-60 ng/mL
Reactivity
Human
Cat no : KE00680
Synonyms
53BP1, FLJ41424, p202, p53 binding protein 1, p53-binding protein 1
Validation Data Gallery
Product Information
KE00680 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TP53BP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TP53BP1. The assay recognizes human TP53BP1. An antibody specific for human TP53BP1 has been pre-coated onto the microwells. The human TP53BP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TP53BP1 is added to detect the captured human TP53BP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TP53BP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.94-60 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7158 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 83%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 31.87 | 1.95 | 6.10 |
| 2 | 8 | 7.07 | 0.32 | 4.50 |
| 3 | 8 | 3.68 | 0.12 | 3.40 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 32.29 | 1.54 | 4.77 |
| 2 | 16 | 6.85 | 0.32 | 4.72 |
| 3 | 16 | 3.61 | 0.13 | 3.59 |
Background Information
TP53BP1 (Tumor Protein P53 Binding Protein 1) is a key protein involved in the DNA damage response (DDR) and repair mechanisms, particularly in the non-homologous end joining (NHEJ) pathway. It plays a crucial role in maintaining genomic stability and has implications in cancer development and treatment. TP53BP1 is ubiquitously expressed in various tissues, including the brain and testis. Its expression levels are significantly higher in some kinds of cancerous tissues, for instance, higher levels of TP53BP1 were observed in non-small cell lung cancer, skin cancer, and cervical cancer specimens.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 53BP1, FLJ41424, p202, p53 binding protein 1, p53-binding protein 1 |