Speedy™ Human/Cynomolgus Monkey TNF beta One-Step ELISA Kit
Sensitivity
1.1 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human/Cynomolgus Monkey
Cat no : SE50234
Synonyms
LTA, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha, TNF β
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey TNF beta One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4049 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human cell culture supernatant | 104% | 103%-106% |
| Cynomolgus monkey cell culture supernatant | 98% | 94%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 245.0 | 2.5 | 1.0 |
| 2 | 8 | 76.0 | 1.3 | 1.7 |
| 3 | 8 | 43.6 | 1.0 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 246.5 | 3.6 | 1.5 |
| 2 | 16 | 75.3 | 1.5 | 2.0 |
| 3 | 16 | 42.7 | 1.4 | 3.3 |
Background Information
TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LTA, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha, TNF β |