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Recombinant Human TIMP4 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3334

Validation Data Gallery

  • Purity of Recombinant Human TIMP4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TIMP4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human TIMP4 protein Cys30-Pro224 (Accession# Q99727) with a rabbit IgG Fc tag at the C-terminus.
GeneID 7079
Accession Q99727
PredictedSize 48.7 kDa
SDS-PAGE 48-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Endogenous inhibitors of matrix metalloproteinases (MMPs) called tissue inhibitors of matrix metalloproteinases (TIMPs) form a family of four members,TIMP1-4. Although possessing many similarities, TIMPs show several structural and biochemical differences, and their expression patterns differ. TIMP4 is characterized by being primarily restricted to cardiovascular structures. Experimental studies have suggested a role for TIMP4 in cardiovascular disorders exhibiting an inflammatory component. Overexpression of TIMP4 has been observed within the arterial wall in rats after a balloon injury. TIMP4 has been shown to suppress vascular smooth muscle cell migration and induce cell apoptosis and to inhibit capillary endothelial cell migration.

References:

1. Baker AH.et al. (2002)J Cell Sci.115:3719-3727. 
2. SpinaleFG.et al.(2002) Circ Res.90:520-530. 
3.LiYY.et al. (1999) Cardiovasc Res.42:162-72. 
4. Schulze CJ.et al. (2003) Circulation.107:2487-92. 
5. Guo YH.et al. (2004)Life Sci.75:2483-93.
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