Human THOC1 ELISA Kit
Sensitivity
0.09 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00863
Synonyms
Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1
Validation Data Gallery
Product Information
KE00863 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human THOC1 ELISA kit is to be used to detect and quantify protein levels of endogenous human THOC1. The assay recognizes human THOC1. An antibody specific for human THOC1 has been pre-coated onto the microwells. The human THOC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human THOC1 is added to detect the captured human THOC1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human THOC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9984 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 80% | 76%-86% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.61 | 0.32 | 3.77 |
| 2 | 8 | 2.21 | 0.13 | 6.01 |
| 3 | 8 | 1.19 | 0.10 | 8.70 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.28 | 0.80 | 8.66 |
| 2 | 16 | 2.24 | 0.12 | 5.25 |
| 3 | 16 | 1.18 | 0.10 | 8.81 |
Background Information
The THO Complex 1 (THOC1) is part of the THO ribonucleoprotein complex that is cotranscriptionally assembled on nascent RNA transcripts, facilitating its interaction with RNA processing and export factors. THOC1 expression is elevated in many human cancers, such as prostate, colorectal and lung cancers , and associated with poor prognosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1 |