Human TGF-beta1 ELISA Kit

Sensitivity

3.3 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00002

Synonyms

1, CED, DPD1, LAP, TGF ?1, TGF beta 1, TGF- beta 1, TGFB, TGFB1, TGFbeta, TGF-beta 1, TGF-beta1, TGFβ1, TGF-β1



Product Information

KE00002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-beta1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-beta1. The assay recognizes human TGF-beta1. An antibody specific for TGF-beta1 has been pre-coated onto the microwells. The TGF-beta1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TGF-beta1 is added to detect the captured TGF-beta1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm. This ELISA kit is cross-reactive with bovine, porcine, equine, and caprine. Therefore, the culture medium should not contain serum components associated with the above species.

Product name Human TGF-beta1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 3.3 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 7040

Recovery

Sample TypeAverageRange
Urine93%86%-101%
Human plasma100%81%-111%
Cell culture supernatants105%93%-115%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120632.628.24.5
22081.23.94.8
32018.50.94.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124659.662.09.4
22483.34.55.5
32415.50.32.0

Background Information

TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-beta appears to promote late stage progression and metastasis in some cancers.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms 1, CED, DPD1, LAP, TGF ?1, TGF beta 1, TGF- beta 1, TGFB, TGFB1, TGFbeta, TGF-beta 1, TGF-beta1, TGFβ1, TGF-β1

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Circ Res

MiR-18a inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.

Authors - Nagore I Marín-Ramos
humancell culture supernatants

Mol Ther

Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells.

Authors - Wei Qiu
humanserum

J Am Soc Nephrol

MicroRNA-23b Targets Ras GTPase-Activating Protein SH3 Domain-Binding Protein 2 to Alleviate Fibrosis and Albuminuria in Diabetic Nephropathy.

Authors - Binghai Zhao
human

Cancer Lett

Small extracellular vesicle-packaged TGFβ1 promotes the reprogramming of normal fibroblasts into cancer-associated fibroblasts by regulating fibronectin in head and neck squamous cell carcinoma.

Authors - Qiang Huang
humancell culture supernatants

Cancer Lett

MiR-26b-5p in small extracellular vesicles derived from dying tumor cells after irradiation enhances the metastasis promoting microenvironment in esophageal squamous cell carcinoma.

Authors - Xiaoyang Yin
humancell culture supernatants

Cancer Lett

NEO212, a conjugate of temozolomide and perillyl alcohol, blocks the endothelial-to-mesenchymal transition in tumor-associated brain endothelial cells in glioblastoma.

Authors - Nagore I Marín-Ramos