KE00202 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TFPI ELISA kit is to be used to detect and quantify protein levels of endogenous TFPI. The assay recognizes human TFPI. An antibody specific for TFPI has been pre-coated onto the microwells. The TFPI protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for TFPI is added to detect the captured TFPI protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.