Human TFEB ELISA Kit
Sensitivity
0.08 ng/mL
Range
0.47-30 ng/mL
Reactivity
Human
Cat no : KE00843
Synonyms
Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35, TCFEB
Validation Data Gallery
Product Information
KE00843 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TFEB ELISA kit is to be used to detect and quantify protein levels of endogenous human TFEB. The assay recognizes human TFEB. An antibody specific for human TFEB has been pre-coated onto the microwells. The human TFEB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TFEB is added to detect the captured human TFEB protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TFEB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7942 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 81% | 74%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 14.89 | 0.60 | 4.04 |
| 2 | 8 | 3.77 | 0.16 | 4.22 |
| 3 | 8 | 1.04 | 0.05 | 4.66 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.91 | 0.61 | 4.12 |
| 2 | 16 | 3.84 | 0.15 | 3.97 |
| 3 | 16 | 1.05 | 0.04 | 4.02 |
Background Information
Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa. TFEB mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus. Fisetin increases the levels of TFEB localized in nuclei.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35, TCFEB |