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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Human Siglec-7/CD328 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1545

Validation Data Gallery

  • Purity of Recombinant Human Siglec-7 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Siglec-7 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Siglec-7 protein Gln19-Leu353 (Accession# Q9Y286-1) with a His tag at the C-terminus.
GeneID 27036
Accession Q9Y286-1
PredictedSize 38.0 kDa
SDS-PAGE 45-65 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs. It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells. Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands .

References:

1. Zheng, Yayun et al. Journal of immunology research vol. 2020 6243819. 
2. Nicoll, G et al. The Journal of biological chemistry vol. 274,48 (1999): 34089-95.
3. Shao, J-Y et al. Scandinavian journal of immunology vol. 84,3 (2016): 182-90.