Human/Mouse SORBS1 ELISA Kit
Sensitivity
0.07 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human/Mouse
Cat no : KE00844
Synonyms
CAP, c-Cbl-associated protein, SH3D5, SORB1, Sorbin and SH3 domain-containing protein 1
Validation Data Gallery
Product Information
KE00844 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse SORBS1 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse SORBS1. The assay recognizes human/mouse SORBS1. An antibody specific for human/mouse SORBS1 has been pre-coated onto the microwells. The human/mouse SORBS1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse SORBS1 is added to detect the captured human/mouse SORBS1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse SORBS1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10580 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 105% | 101%-108% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 25.35 | 1.35 | 5.33 |
| 2 | 8 | 6.59 | 0.15 | 2.31 |
| 3 | 8 | 1.98 | 0.06 | 3.21 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 27.41 | 2.78 | 10.15 |
| 2 | 16 | 6.92 | 0.42 | 6.10 |
| 3 | 16 | 2.11 | 0.15 | 7.31 |
Background Information
The sorbin homology (SoHo) family of adapter and scaffold proteins consists of three proteins: CAP, also known as Sorbin and SH3 domain containing 1 (Sorbs1), ArgBP2, also known as Sorbs2, and Vinexin, also known as Sorbs3. All Sorbs proteins are highly expressed in heart tissue, skeletal muscle, adipose tissue, and cells of the immune system, functioning as SH3-domain-mediated adaptors of scaffolding molecules. SORBS1 can bind to signaling molecules (e.g., c-Cbl, c-Abl, and insulin reporter) to regulate glucose transport, transcription activity, and insulin signaling.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CAP, c-Cbl-associated protein, SH3D5, SORB1, Sorbin and SH3 domain-containing protein 1 |