Human SNAT2 ELISA Kit
Sensitivity
0.4 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00475
Synonyms
SLC38A2,ATA2,KIAA1382,PRO1068,Protein 40 9 1
Validation Data Gallery
Product Information
KE00475 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SNAT2 ELISA kit is to be used to detect and quantify protein levels of endogenous human SNAT2. The assay recognizes human SNAT2. An antibody specific for human SNAT2 has been pre-coated onto the microwells. The human SNAT2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SNAT2 is added to detect the captured human SNAT2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SNAT2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54407 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 88%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 940.6 | 57.2 | 6.1 |
| 2 | 8 | 237.2 | 5.3 | 2.2 |
| 3 | 8 | 119.2 | 4.9 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 923.0 | 62.1 | 6.7 |
| 2 | 16 | 226.3 | 14.6 | 6.5 |
| 3 | 16 | 114.2 | 7.1 | 6.2 |
Background Information
Sodium-coupled neutral amino acid transporter 2 (SNAT2) is a member of the system A family of transporters that primarily mediates the sodium-dependent uptake of small neutral amino acids including alanine and glutamine into cells. The sodium-coupled neutral amino acid transporter 2 (SNAT2) is involved in maintaining the cellular metabolic status by influencing the amino acid content of the cell, which in turn determines the overall size and composition of the intracellular amino acid pool.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SLC38A2,ATA2,KIAA1382,PRO1068,Protein 40 9 1 |