Human SKP2 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat no : KE00979
Synonyms
Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, FBL1, F-box protein Skp2
Validation Data Gallery
Product Information
KE00979 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SKP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human SKP2. The assay recognizes human SKP2. An antibody specific for human SKP2 has been pre-coated onto the microwells. The human SKP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SKP2 is added to detect the captured human SKP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SKP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6502 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 89%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 19.78 | 1.50 | 7.59 |
| 2 | 8 | 4.99 | 0.30 | 5.96 |
| 3 | 8 | 2.57 | 0.24 | 9.22 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 18.59 | 2.10 | 11.32 |
| 2 | 16 | 4.77 | 0.37 | 7.72 |
| 3 | 16 | 2.46 | 0.30 | 12.11 |
Background Information
SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B. The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, FBL1, F-box protein Skp2 |