Human P-selectin ELISA Kit

Sensitivity

4.7 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Human

Cat no : KE00064

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selecin, SELP



Product Information

KE00064 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SELP ELISA kit is to be used to detect and quantify protein levels of endogenous SELP. The assay recognizes human SELP. An antibody specific for SELP has been pre-coated onto the microwells. The SELP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for SELP is added to detect the captured SELP protein. For signal development, Streptavidin-HRP is added is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human P-selectin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 4.7 pg/mL
Range 62.5-4000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Serum97%78%-107%
Human plasma100%85%-109%
Cell culture supernatants95%84%-113%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,878.546.12.5
220483.914.93.1
320118.66.25.2

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,983.375.93.8
224479.618.63.9
324104.66.46.1

Background Information

SELP, also named as P-selectin, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selecin, SELP

Publications

SpeciesSample TypeTitle
humanplasma

Intern Med

Nocturnal Hypoxemia and High Circulating TNF-α Levels in Chronic Thromboembolic Pulmonary Hypertension.

Authors - Akira Naito