Human SALL4 ELISA Kit
Sensitivity
2.6 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00348
Synonyms
dJ1112F19.1, DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797
Validation Data Gallery
Product Information
KE00348 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SALL4 ELISA kit is to be used to detect and quantify protein levels of endogenous human SALL4. The assay recognizes human SALL4. An antibody specific for human SALL4 has been pre-coated onto the microwells. The human SALL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SALL4 is added to detect the captured human SALL4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SALL4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57167 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 77%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,220.4 | 72.4 | 5.9 |
| 2 | 20 | 336.6 | 22.0 | 6.5 |
| 3 | 20 | 74.8 | 4.41 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,252.4 | 111.4 | 8.9 |
| 2 | 24 | 328.6 | 18.9 | 5.8 |
| 3 | 24 | 74.6 | 6.2 | 8.3 |
Background Information
Spalt-Like Transcription Factor 4 (SALL4) is a zinc-finger transcriptional factor for embryonic stem cell self-renewal and pluripotency. SALL4 overexpression is found in several cancer tissues and hematologic malignancies,suggesting a key role of SALL4 in cancer onset and progression. Reactivation of SALL4 in cancerous cells is a key event in tumorigenesis and confers malignancy features to cells. The SALL4 protein contains several zinc finger cluster (ZFC) domains spread in different parts of the protein which give it both transcription activation and repression functions. SALL4 has two isoforms. SALL4A originates from full length transcript, while SALL4B as a spliced isoform lacks a part of exon 2. SALL4 abundancy is regulated by transcriptional and post-transcriptional mechanisms. Both SALL4A and SALL4B isoforms can bind β-catenin protein and synergistically activate the Wnt/β-catenin signaling pathway.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | dJ1112F19.1, DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797 |