Speedy™ Human Resistin One-Step ELISA Kit
Sensitivity
0.3 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : SE50243
Synonyms
RETN, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Resistin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56729 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 114% | 110%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,536.7 | 113.0 | 4.5 |
| 2 | 8 | 618.1 | 27.4 | 4.4 |
| 3 | 8 | 263.8 | 12.9 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,431.6 | 147.8 | 6.1 |
| 2 | 16 | 614.7 | 30.9 | 5.0 |
| 3 | 16 | 264.8 | 12.0 | 4.5 |
Background Information
Resistin, a cysteine-rich hormone secreted primarily by rodent fat cells, was postulated to be implicated in obesity, insulin resistance and diabetes. Resistin is also secreted by macrophages in humans, promoting inflammation. Resistin modulates the synthesis and secretion of key pro-inflammatory cytokines such as TNF-α, IL-6 and IL12 in macrophages via a nuclear factor-kB (NF-kB)-dependent pathway. Given the connection between inflammation and cancer, resistin was soon considered for its role in cancer. There were observations supporting its possible use as a diagnostic marker, expression levels of which also seemed to differ in patients with varying clinical stages. Resistin has been also linked to other pathologies such as atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RETN, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2 |