Recombinant Human Coagulation factor II protein (His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg1337
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Coagulation factor II protein Ala44-Glu622 (Accession# P00734) with a His tag at the C-terminus. |
| GeneID | 2147 |
| Accession | P00734 |
| PredictedSize | 66.1 kDa |
| SDS-PAGE | 68-90 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Coagulation factors are a group of related proteins that are essential for normal blood clotting (haemostasis). After an injury, clots protect the body by sealing off damaged blood vessels and preventing further blood loss. Prothrombin circulates in the bloodstream in an inactive form until an injury occurs. In response to that, prothrombin is converted to its active form, thrombin. Thrombin next converts a protein called fibrinogen into fibrin. Thrombin is also important for cell growth and division, tissue repair, and new blood vessels formation - angiogenesis.
References:
1.Akhavan, S et al.Thrombosis and haemostasis vol. 84,6 (2000): 989-97. 2.Danckwardt, Sven et al. Acta haematologica vol. 115,3-4 (2006): 192-7. 3.Jayandharan, G et al. Journal of thrombosis and haemostasis : JTH vol. 3,7 (2005): 1446-53. 4.McGlennen, Ronald C, and Nigel S Key.Archives of pathology & laboratory medicine vol. 126,11 (2002): 1319-25.