Human PTPRS ELISA Kit
Sensitivity
1.7 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00485
Synonyms
EC:3.1.3.48, PTPSIGMA, R PTP S, R PTP sigma, Receptor-type tyrosine-protein phosphatase S
Validation Data Gallery
Product Information
KE00485 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PTPRS ELISA kit is to be used to detect and quantify protein levels of endogenous human PTPRS. The assay recognizes human PTPRS. An antibody specific for human PTPRS has been pre-coated onto the microwells. The human PTPRS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PTPRS is added to detect the captured human PTPRS protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PTPRS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cerebrospinal fluid |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5802 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 99%-114% |
| Human cerebrospinal fluid (CSF) | 100% | 96%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 484.9 | 30.9 | 6.4 |
| 2 | 8 | 115.2 | 4.8 | 4.1 |
| 3 | 8 | 53.8 | 1.4 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 476.9 | 28.7 | 6.0 |
| 2 | 16 | 113.3 | 4.2 | 3.7 |
| 3 | 16 | 57.5 | 4.1 | 7.1 |
Background Information
Type II a receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair.The expression of rPTPσ has previously been reported in b-cells and other target organs for INS although the probes chosen did not permit to distinguish between the splice variants.Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ,and the short splice variants rPTPσ 3 and 4 contain an E-domain of 95 kDa (PMID: 16552719). rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75-80kDa immunoreactive band (PMID: 19780196).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.1.3.48, PTPSIGMA, R PTP S, R PTP sigma, Receptor-type tyrosine-protein phosphatase S |