Human PSMC3IP ELISA Kit
Sensitivity
3.6 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00786
Synonyms
GT198, Homologous-pairing protein 2 homolog, HOP2, Proteasome 26S ATPase subunit 3-interacting protein, PSMC3 interacting protein
Validation Data Gallery
Product Information
KE00786 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PSMC3IP ELISA kit is to be used to detect and quantify protein levels of endogenous human PSMC3IP. The assay recognizes human PSMC3IP. An antibody specific for human PSMC3IP has been pre-coated onto the microwells. The human PSMC3IP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PSMC3IP is added to detect the captured human PSMC3IP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PSMC3IP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 29893 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 82%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,223.7 | 62.9 | 5.1 |
| 2 | 8 | 302.9 | 7.9 | 2.6 |
| 3 | 8 | 101.5 | 3.5 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,217.2 | 68.3 | 5.6 |
| 2 | 16 | 314.7 | 14.6 | 4.7 |
| 3 | 16 | 102.2 | 5.0 | 4.9 |
Background Information
PSMC3IP (PSMC3-interacting protein), also known as TBPIP, GT198, HUMGT198A, or Hop2, is a nuclear protein that plays important roles in meiotic recombination, DNA repair, transcriptional regulation, cancer development, and cell cycle regulation. PSMC3IP is a subunit of the PSMC3IP/MND1 complex, interacting with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. Additionally, PSMC3IP acts as a coactivator of ligand-dependent transcription, participating in transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in the PSMC3IP gene can lead to XX female gonadal dysgenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GT198, Homologous-pairing protein 2 homolog, HOP2, Proteasome 26S ATPase subunit 3-interacting protein, PSMC3 interacting protein |