Human PPP1R15B ELISA Kit
Sensitivity
0.03 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00666
Synonyms
CREP, FLJ14744
Validation Data Gallery
Product Information
KE00666 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PPP1R15B ELISA kit is to be used to detect and quantify protein levels of endogenous human PPP1R15B. The assay recognizes human PPP1R15B. An antibody specific for human PPP1R15B has been pre-coated onto the microwells. The human PPP1R15B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PPP1R15B is added to detect the captured human PPP1R15B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PPP1R15B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 84919 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 94%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.91 | 0.23 | 4.72 |
| 2 | 8 | 1.34 | 0.12 | 8.99 |
| 3 | 8 | 0.36 | 0.02 | 5.18 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.96 | 0.30 | 6.13 |
| 2 | 16 | 1.39 | 0.10 | 7.34 |
| 3 | 16 | 0.36 | 0.04 | 9.89 |
Background Information
Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of the PPP1R15 family. It forms a complex with protein phosphatase 1 (PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells; thus, it is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is associated with endoplasmic reticulum (ER) stress response, resulting in a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and, more modestly, against the accumulation of malfolded proteins in the ER. Allelic variants of PPP1R15B are associated with microcephaly, short stature, and impaired glucose metabolism.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CREP, FLJ14744 |