Recombinant Human POFUT1 protein (C-rFc)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6687
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human POFUT1 protein Gly27-Phe388 (Accession# Q9H488-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 23509 |
| Accession | Q9H488-1 |
| PredictedSize | 66.9 kDa |
| SDS-PAGE | 65-80 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.
References:
1.Monno, Masayoshi et al. Cell biochemistry and function vol. 42,2 (2024): e3989. 2.Li, Qi et al. J Bioenerg Biomembr. 2021;53(5):621-632. 3.Matsumoto, Kenjiroo et al. Glycobiology. 2024;34(8):cwae047.