Recombinant Human PMCH protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2978
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human PMCH protein Ile22-Val165 (Accession# P20382) a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 5367 |
| Accession | P20382 |
| PredictedSize | 42.4 kDa |
| SDS-PAGE | 44-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
The orexigenic neuropeptide melanin-concentrating hormone (MCH), a product of PMCH, is an important mediator of energy homeostasis. PMCH is expressed predominantly in neurons of the lateral hypothalamic area and the incerto hypothalamic area, which project throughout the brain. PMCH is also expressed in some peripheral tissues, such as the testes, although at lower levels than in the brain. PMCH plays an important role in determining energy balance during early development.
References:
1.Mul JD. et al. (2010) Am J Physiol Endocrinol Metab. 298(3):E477-E488. 2.Mul JD. et al. (2013) PLoS One. 8(3):e60214. 3.Bertolesi GE. et al. (2019) Pigment Cell Melanoma Res. 32(4):510-527.