Human PEBP4 ELISA Kit
Sensitivity
0.11 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00389
Synonyms
PEBP4,CORK 1,CORK1,GWTM1933,hPEBP4
Validation Data Gallery
Product Information
KE00389 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PEBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous human PEBP4. The assay recognizes human PEBP4. An antibody specific for human PEBP4 has been pre-coated onto the microwells. The human PEBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PEBP4 is added to detect the captured human PEBP4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PEBP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 157310 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 110% | 88%-124% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 10.67 | 0.50 | 4.71 |
| 2 | 20 | 2.53 | 0.08 | 3.23 |
| 3 | 20 | 0.67 | 0.04 | 6.49 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 11.19 | 0.53 | 4.77 |
| 2 | 24 | 2.47 | 0.16 | 6.38 |
| 3 | 24 | 0.61 | 0.06 | 9.59 |
Background Information
PEBP4 (Phosphatidylethanolamine binding protein 4) is a multifunctional protein that belongs to a small family (PEBP1-4) in mammalian cells. PEBP4 is evolutionally divergent from the other three forms that share almost 80% identity and more than 90% similarity, suggesting that PEBP4 is functionally different from other forms. At cellular levels, it has been shown that the knockdown of PEBP4 in cancer cells suppresses cell proliferation, migration, and invasion, leading to their apoptosis. In contrast, overexpression of PEBP4 induces opposite changes and confers resistance to chemotherapy and radiotherapy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PEBP4,CORK 1,CORK1,GWTM1933,hPEBP4 |