Human PDCD4 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00635
Synonyms
Dug, H731, Nuclear antigen H731-like, PDCD 4, Tis
Validation Data Gallery
Product Information
KE00635 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PDCD4 ELISA kit is to be used to detect and quantify protein levels of endogenous human PDCD4. The assay recognizes human PDCD4. An antibody specific for human PDCD4 has been pre-coated onto the microwells. The human PDCD4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PDCD4 is added to detect the captured human PDCD4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PDCD4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 27250 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 79% | 77%-82% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.29 | 0.80 | 7.81 |
| 2 | 8 | 2.45 | 0.04 | 1.63 |
| 3 | 8 | 1.18 | 0.08 | 6.47 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.77 | 1.04 | 10.65 |
| 2 | 16 | 2.41 | 0.10 | 4.36 |
| 3 | 16 | 1.24 | 0.08 | 6.43 |
Background Information
Human PDCD4 was first found as a nuclear antigen gene and mapped at chromosome 10q24 . The expression of the gene was modulated by interleukins such as IL-2, IL-12, and IL-15. PDCD4 expresses ubiquitously in the tissues and the protein is localized in the nuclei or cytoplasm or in both depending on the cell types. The expression of PDCD4-protein is often down-regulated in the tumors but is maintained in high levels in a large number of tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Dug, H731, Nuclear antigen H731-like, PDCD 4, Tis |