Speedy™ Human PD-L1/CD274 One-Step ELISA Kit
Sensitivity
2.8 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50035
Synonyms
CD274, PD-L1, B7 H1, B7-H1, hPD-L1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human PD-L1/CD274 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 29126 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 97% | 79%-123% |
| Cell culture supernatant | 115% | 100%-129% |
| Urine | 107% | 93%-112% |
| Cell lysate | 110% | 105%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 546.3 | 13.4 | 2.5 |
| 2 | 8 | 127.0 | 0.5 | 0.4 |
| 3 | 8 | 65.2 | 0.7 | 1.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 569.8 | 26.7 | 4.7 |
| 2 | 16 | 129.2 | 2.8 | 2.2 |
| 3 | 16 | 67.2 | 2.4 | 3.6 |
Background Information
PD-L1 (programmed cell death ligand 1, also known as CD274 or B7-H1) is a 290 aa type I transmembrane protein. PD-L1 is expressed constitutively on T cells, B cells, DCs, macrophages, mesenchymal stem cells and cultured bone marrow-derived mast cells. In addition, PD-L1 is also expressed on many nonhematopoietic cell types, including vascular endothelial cells, epithelial cells, muscle cells, hepatocytes, pancreatic islet cells, astrocytes in the brain, placental syncytiotrophoblasts, and cells in cornea, iris-ciliary body and retina of eye. PD-L1 is frequently upregulated in a wide variety of solid tumors, including melanoma, ovarian, lung, glioblastoma, breast, and pancreatic cancers. PD-L1 and PD-L2 are two ligands of PD-1. Engagement of PD-1 by PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection. Besides the membrane-bound form, PD-L1 can also exist as a soluble form (sPD-L1) generated either by proteolytic cleavage of membrane-bound form or by translation of alternative spliced mRNA.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD274, PD-L1, B7 H1, B7-H1, hPD-L1 |