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Recombinant Human Osteopontin protein (hFc Tag)

Species

Human

Purity

>80 %, SDS-PAGE

Tag

hFc Tag

Activity

EC50: 0.14 -0.56 μg/mL

Cat no : Eg0696

Validation Data Gallery

  • Purity of Recombinant Human Osteopontin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Osteopontin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human ITGAV (GST tag) at 1 μg/mL (100 μL/well) can bind Human Osteopontin (hFc tag) with a linear range of 0.14 -0.56 μg/mL.

    Immobilized Human ITGAV (GST tag) at 1 μg/mL (100 μL/well) can bind Human Osteopontin (hFc tag) with a linear range of 0.14 -0.56 μg/mL.

Product Information

Purity >80 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human ITGAV (GST tag) at 1 μg/mL (100 μL/well) can bind Human Osteopontin (hFc tag) with a linear range of 0.14 -0.56 μg/mL.
Expression HEK293-derived Human Osteopontin protein Ile17-Asn300 (Accession# P10451-5) with a human IgG1 Fc tag at the C-terminus.
GeneID 6696
Accession P10451-5
PredictedSize 59.5 kDa
SDS-PAGE 85-95 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone. OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. It can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

References:

1. Denhardt DT, et al. (2001). J Clin Invest. 107(9):1055-61.
2. Fisher LW, et al. (2001). Biochem Biophys Res Commun. 280(2):460-5.
3. Rittling SR, et al. (2004). Br J Cancer. 90(10):1877-81.
4. Rangaswami H, et al. (2006). Trends Cell Biol. 16(2):79-87.

Publications

SpeciesTitle
human

Sci Rep

IL-1β alleviates suppressive potential of bone marrow mesenchymal stem cells in macrophage M1 polarization via autophagy mediated degradation of SPP1.

Authors - Peng Ye
View Article
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