Speedy™ Human Nectin-4 One-Step ELISA Kit
Sensitivity
0.8 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50058
Synonyms
PVRL4, Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin cell adhesion molecule 4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Nectin-4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81607 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 111% | 103%-117% |
| Cell culture supernatant | 108% | 98%-119% |
| Urine | 101% | 97%-106% |
| Cell lysate | 107% | 102%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 515.5 | 30.8 | 6.0 |
| 2 | 8 | 122.3 | 3.8 | 3.1 |
| 3 | 8 | 36.7 | 1.1 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 496.1 | 29.5 | 5.9 |
| 2 | 16 | 119.6 | 4.1 | 3.4 |
| 3 | 16 | 35.6 | 1.5 | 4.2 |
Background Information
Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion. The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction. It acts as a receptor for measles virus. Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PVRL4, Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin cell adhesion molecule 4 |