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Recombinant Human Nectin-1/PVRL1 protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1707

Validation Data Gallery

  • Purity of Recombinant Human Nectin-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Nectin-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human Nectin-1/PVRL1 was greater than 90% as determined by SEC-HPLC.

    The purity of Human Nectin-1/PVRL1 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Nectin-1 protein Gln31-Thr334 (Accession# Q15223-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 5818
Accession Q15223-1
PredictedSize 60.2 kDa
SDS-PAGE 65-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight (PMID: 12376527; 15731277).

References:

1. Takai Y. et al. (2003). Cancer Sci. 94(8):655-667.
2. Barron MJ. et al. (2008). Hum Mol Genet. 17(22):3509-3520.
3. Geraghty RJ. et al. (1998). Science. 280(5369):1618-1620.
4. Kim DY. et al. (2002). J Biol Chem. 277(51):49976-49981.
5. Struyf F. et al. (2005). J Virol. 79(6):3841-3845.
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