Product Information
Natural Killer cells, also as known as NK cells, are a type of cytotoxic lymphocyte belong to the innate immune system which are important to adaptive immune response in vertebrate. Around 5-20% of all circulating lymphocytes are NK cells in human. Human NK Cell Isolation kit is designed to isolate untouched NK cells which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non-NK cells will be retained in the tube by magnetic separator. The untouched NK cells are collected in supernatant. Any downstream application can be performed on NK cells after separation, such as Flow Cytometry and in vitro culture.
Components
KMS308-10:
·MS308A-10: 300 µL Human NK Cell Isolation Biotin Antibody Cocktail (CD3, CD4, CD8, CD19, CD14, CD15, CD279, CD36, CD235a, CD123)
·MS308B-10: 300µL streptavidin magnetic beads
KMS308-100:
·MS308A-100: 3mL Human NK Cell Isolation Biotin Antibody Cocktail (CD3, CD4, CD8, CD19, CD14, CD15, CD279, CD36, CD235a, CD123)
·MS308B-100: 3mL streptavidin magnetic beads
Storage buffer
2-8°C
Usage
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
Reactivity
Human
Beads diameter
2.7 µm
Recommend usage
30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1x107 cells
Cited in Article as
kms308, Human NK Cell Isolation Kit, Proteintech, IL, USA
Documentation
| Datasheet |
|---|
| Human NK Cell Isolation Kit KMS308 datasheet |