Speedy™ Human NGAL One-Step ELISA Kit
Sensitivity
7.1 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50023
Synonyms
LCN2, lipocalin 2, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL
Validation Data Gallery
Product Information
| Product name | Speedy™ Human NGAL One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 7.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3934 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 88% | 81%-95% |
| Cell culture supernatant | 98% | 84%-109% |
| Urine | 99% | 95%-107% |
| Saliva | 97% | 90%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 598.1 | 13.1 | 2.2 |
| 2 | 8 | 221.8 | 8.9 | 4.0 |
| 3 | 8 | 96.6 | 1.6 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 599.1 | 13.1 | 2.2 |
| 2 | 16 | 220.8 | 6.7 | 3.0 |
| 3 | 16 | 95.3 | 2.4 | 2.5 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL was identified, including iron trafficking, chemotactic, and promote cell differentiation and proliferation. NGAL was originally found in human neutrophils, and expressed in tissues containing renal, uterus, liver, spleen, as well as cardiomyocytes, neurons and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation and metabolic diseases, and it can be detected in human serum, saliva and urine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LCN2, lipocalin 2, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL |