Human NGAL ELISA Kit

Sensitivity

3.2 pg/mL

Range

19.5-1250 pg/mL

Reactivity

Human

Cat no : KE00327

Synonyms

24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25



Product Information

KE00327 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous NGAL. The assay recognizes human NGAL. An antibody specific for NGAL has been pre-coated onto the microwells. The NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human NGAL ELISA Kit
Tests 1 X 96 well plate
Sample type Human plasma,Cell culture supernatants,Urine,Saliva
Assay type Sandwich
Sensitivity 3.2 pg/mL
Range 19.5-1250 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3934

Recovery

Sample TypeAverageRange
Cell culture supernatants92%81%-106%
Urine91%78%-105%
Saliva98%87%-118%
Human plasma93%80%-103%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120542.825.04.6
220125.58.66.8
32030.32.37.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124572.637.16.5
224135.010.27.5
32432.12.88.8

Background Information

Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL was identified, including iron trafficking, chemotactic, and promote cell differentiation and proliferation. NGAL was originally found in human neutrophils, and expressed in tissues containing renal, uterus, liver, spleen, as well as cardiomyocytes, neurons and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation and metabolic diseases, and it can be detected in human serum, saliva and urine.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms 24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25