Human NCR3/NKp30 ELISA Kit
Sensitivity
0.7 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00802
Synonyms
NCR3, 1C7, CD337, LY117, MALS
Validation Data Gallery
Product Information
KE00802 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NCR3/NKp30 ELISA kit is to be used to detect and quantify protein levels of endogenous human NCR3/NKp30. The assay recognizes human NCR3/NKp30. An antibody specific for human NCR3/NKp30 has been pre-coated onto the microwells. The human NCR3/NKp30 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NCR3/NKp30 is added to detect the captured human NCR3/NKp30 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NCR3/NKp30 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 259197 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 93%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,132.8 | 26.1 | 2.3 |
| 2 | 8 | 269.4 | 5.4 | 2.0 |
| 3 | 8 | 131.2 | 4.2 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,091.9 | 65.0 | 6.0 |
| 2 | 16 | 251.1 | 20.7 | 8.2 |
| 3 | 16 | 131.1 | 6.0 | 4.6 |
Background Information
NKp30 (CD337) (NCR3) is a 30kDa type I trans-membrane protein characterized by a single V-type immunoglobulin (Ig) extracellular domain. NKp30 expression is mostly restricted to NK cells although recent studies found this NCR to be present on the surface of additional immune cells, including γδ T cells and Innate Lymphoid Cells (ILCs). NKp30 is involved in NK cell-mediated killing of various tumor cell lines, viral infected cells and also of autologous dendritic cells (DCs) during the so called "editing" process.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NCR3, 1C7, CD337, LY117, MALS |