Human NCALD ELISA Kit
Sensitivity
3 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00028
Synonyms
NCALD, neurocalcin delta
Validation Data Gallery
Product Information
KE00028 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NCALD ELISA kit is to be used to detect and quantify protein levels of endogenous NCALD. The assay recognizes human NCALD. A polyclonal antibody specific for NCALD has been pre-coated onto the microwells. The NCALD protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for NCALD is added to detect the captured NCALD protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human NCALD ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum |
| Assay type | Sandwich |
| Sensitivity | 3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 83988 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 105% | 96%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
NCALD belongs to the recoverin family. And it is a member of the EF-hand calcium-binding protein superfamily. NCALD may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. It plays an important role in the regulation of the neuronal signal transduction process. NCALD is a likely candidate for conferring susceptibility to diabetic nephropathy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NCALD, neurocalcin delta |