Human N-cadherin ELISA Kit Elisa Kit KE00114

Images See all

概要

製品名:

Human N-cadherin ELISA Kit

試験方法:

1 X 96 well plate

測定サンプル対象:

Serum, Plasma, Cell culture supernatants

アッセイ方法:

Sandwich

感度:

5.6 pg/mL

測定範囲:

46.88-3000 pg/mL

測定可能動物種:

Human

適用アプリケーション:

Sandwich ELISA

回収率:

Sample Type	Average	Range
Cell culture supernatants	96%	85%-107%
Human serum	99%	77%-117%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	1,413.1	48.5	3.4
2	20	395.9	11.8	3.3
3	20	91.9	3.9	4.3

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	1,347.9	72.2	5.4
2	24	348.7	13.6	3.9
3	24	89.0	3.1	3.5

製品概要

KE00114 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The N-caderin ELISA kit is to be used to detect and quantify protein levels of endogenous N-caderin. The assay recognizes human N-caderin. A monoclonal antibody specific for N-caderin has been pre-coated onto the microwells. The N-caderin protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for human N-caderin is added to detect the captured human N-caderin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性

保存方法:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

背景説明

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes. N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity. Soluble N-cadherin (sN-CAD) is found in biological fluids, including serum, seminal fluid samples, and urine. It has been shown that significantly higher amounts of serum sN-CAD are present in cancer patients than in persons with no evidence of disease.

Human N-cadherin ELISA Kit0 Publications

カタログ番号: KE00114

74,000円

（希望販売価格）

概要

製品特性

背景説明

Showing 1- [0] of [0] Publications