Human/Mouse/Rat EZH2 ELISA Kit
Sensitivity
0.19 ng/mL
Range
0.47-30 ng/mL
Reactivity
Human/Mouse/Rat
Cat no : KE00839
Synonyms
EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1
Validation Data Gallery
Product Information
KE00839 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat EZH2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat EZH2. The assay recognizes human/mouse/rat EZH2. An antibody specific for human/mouse/rat EZH2 has been pre-coated onto the microwells. The human/mouse/rat EZH2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat EZH2 is added to detect the captured human/mouse/rat EZH2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat EZH2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.19 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2146 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 82% | 80%-85% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.76 | 0.35 | 2.54 |
| 2 | 8 | 5.25 | 0.12 | 2.34 |
| 3 | 8 | 3.44 | 0.04 | 1.14 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 13.09 | 0.76 | 5.83 |
| 2 | 16 | 5.11 | 0.18 | 3.60 |
| 3 | 16 | 3.33 | 0.14 | 4.11 |
Background Information
Enhancer of zeste homolog 2 (EZH2) is an evolutionary conserved gene identified in many species, sharing similar structural motifs and domains. As a histone methyltransferase, EZH2 has been identified as a catalytic subunit of PRC2 for tri-methylation of histone H3 at Lys 27 (H3K27me3) by SET domain in its C-terminus, which silences targeted genes and is involved in various biological functions (e.g. cell cycle, cell proliferation, cell differentiation, etc.). The role of EZH2 in cancer progression was also considered and unraveled. EZH2 is aberrantly overexpressed in various malignant tumors, such as prostate cancer, breast cancer, and ovarian cancer. EZH2 mediates H3K27me3 and functions as a critical factor in promoting tumor growth and metastasis in many malignant tumor models.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1 |