Human/Mouse MYL3 ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human/Mouse
Cat no : KE00643
Synonyms
MLC1V, MLC-lV/sb, MYL 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, Myosin light chain 3
Validation Data Gallery
Product Information
KE00643 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MYL3 ELISA kit is to be used to detect and quantify protein levels of endogenous human MYL3. The assay recognizes human MYL3. An antibody specific for human MYL3 has been pre-coated onto the microwells. The human MYL3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MYL3 is added to detect the captured human MYL3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse MYL3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4634 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 74% | 71%-76% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.44 | 0.27 | 2.0 |
| 2 | 8 | 2.44 | 0.09 | 3.8 |
| 3 | 8 | 1.31 | 0.06 | 4.2 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 13.82 | 0.55 | 4.0 |
| 2 | 16 | 2.49 | 0.17 | 6.8 |
| 3 | 16 | 1.37 | 0.07 | 5.4 |
Background Information
MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MLC1V, MLC-lV/sb, MYL 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, Myosin light chain 3 |