Human MCP-1 ELISA Kit

Sensitivity

6.9 pg/mL

Range

31.25-1000 pg/mL

Reactivity

Human

Cat no : KE00091

Synonyms

CCL2, GDCF 2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC CF



Product Information

KE00091 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MCP-1. The assay recognizes human MCP-1. A monoclonal antibody specific for MCP-1 has been pre-coated onto the microwells. The MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for MCP-1 is added to detect the captured MCP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human MCP-1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 6.9 pg/mL
Range 31.25-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma90%82%-103%
Cell culture supernatants102%84%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,099.527.92.5
220295.116.75.7
32042.53.99.4

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,075.141.03.8
224259.819.37.4
32440.33.69.0

Background Information

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CCL2, GDCF 2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC CF

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
humancell culture supernatants

Int J Mol Sci

Abiotrophia defectiva DnaK Promotes Fibronectin-Mediated Adherence to HUVECs and Induces a Proinflammatory Response.

Authors - Minoru Sasaki

Exp Clin Endocrinol Diabetes

Role of the Specialized Pro-resolving Mediator Resolvin D1 in Hashimoto's Thyroiditis.

Authors - Jing Song
humancell culture supernatants

J Cell Biochem

Combined application of geranylgeranylacetone and amelogenin promotes angiogenesis and wound healing in human periodontal ligament cells.

Authors - Hiroaki Yamato
humancell culture supernatants

Ecotoxicol Environ Saf

In vitro evaluation of nanoplastics using human lung epithelial cells, microarray analysis and co-culture model.

Authors - Sheng Yang
humanserum

J Immunol

Macrophagic Extracellular Vesicle CXCL2 Recruits and Activates the Neutrophil CXCR2/PKC/NOX4 Axis in Sepsis.

Authors - Guozhen Wang