Recombinant Human MIF protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4501
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human MIF protein Pro2-Ala115 (Accession# P14174) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 4282 |
| Accession | P14174 |
| PredictedSize | 38.6 kDa |
| SDS-PAGE | 40-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 20 mM PB, 150 mM NaCl, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Macrophage migration inhibitory factor (MIF) is a homo-trimeric protein that acts as a pleiotropic pro-inflammatory cytokine. It contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. It is involved in multiple functions including leukocyte recruitment, inflammation, immune responses, cell proliferation, tumourigenesis and glucocorticoid (GC) counterregulation.
References:
1.Lue H. et al. (2002) Microbes Infect. 4(4):449-60. 2.Martin J. et al. (2009) FASEB J. 23(3):720-30. 3.Bucala, R. (1996) FASEB J. 10(14):1607-13. 4.Gore Y. et al. (2008) J Biol Chem. 283(5):2784-92.