Human MELK ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00482
Synonyms
MELK,EC:2.7.10.2,EC:2.7.11.1,hMELK,hPK38
Validation Data Gallery
Product Information
KE00482 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MELK ELISA kit is to be used to detect and quantify protein levels of endogenous human MELK. The assay recognizes human MELK. An antibody specific for human MELK has been pre-coated onto the microwells. The human MELK protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MELK is added to detect the captured human MELK protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MELK ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9833 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 78% | 74%-80% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.66 | 0.28 | 5.92 |
| 2 | 8 | 1.22 | 0.06 | 4.93 |
| 3 | 8 | 0.59 | 0.06 | 9.78 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.58 | 0.35 | 7.59 |
| 2 | 16 | 1.17 | 0.08 | 6.90 |
| 3 | 16 | 0.61 | 0.05 | 8.24 |
Background Information
MELK is a serine/threonine kinase involved in various physiological and pathological processes, including cell proliferation, cell cycle regulation, and apoptosis inhibition. In cancer biology, MELK has emerged as a significant oncogenic factor. It is highly expressed in various cancers, such as triple-negative breast cancer, glioblastoma, colorectal cancer, lung cancer, and gastric cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MELK,EC:2.7.10.2,EC:2.7.11.1,hMELK,hPK38 |