Speedy™ Human/Cynomolgus Monkey MCP-1/CCL2 One-Step ELISA Kit
Sensitivity
0.5 pg/mL
Range
3.13-200 pg/mL
Reactivity
Human/Cynomolgus Monkey
Cat no : SE50041
Synonyms
CCL2, MCP1, MCP-1, C C motif chemokine 2, C-C motif chemokine 2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey MCP-1/CCL2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6347 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 97%-102% |
| Human cell culture supernatant | 100% | 100%-101% |
| Cynomolgus monkey serum | 97% | 85%-112% |
| Cynomolgus monkey cell culture supernatant | 88% | 82%-94% |
| Human urine | 94% | 88%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 115.7 | 4.3 | 3.7 |
| 2 | 8 | 27.3 | 0.7 | 2.4 |
| 3 | 8 | 13.5 | 0.5 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 110.3 | 6.4 | 5.8 |
| 2 | 16 | 26.3 | 1.3 | 5.0 |
| 3 | 16 | 12.9 | 0.8 | 6.4 |
Background Information
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL2, MCP1, MCP-1, C C motif chemokine 2, C-C motif chemokine 2 |