Speedy™ Human Leptin One-Step ELISA Kit
Sensitivity
10.1 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : SE50012
Synonyms
LEP, OB, Obese protein, Obesity factor, OBS
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Leptin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 10.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3952 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 109% | 98%-124% |
| Human plasma | 101% | 82%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 972.2 | 19.0 | 2.0 |
| 2 | 8 | 228.6 | 2.6 | 1.2 |
| 3 | 8 | 101.2 | 4.6 | 4.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 976.0 | 26.7 | 2.7 |
| 2 | 16 | 234.0 | 13.2 | 5.6 |
| 3 | 16 | 101.2 | 3.7 | 3.6 |
Background Information
Leptin is a product of the obese (ob) gene and, following synthesis and secretion from fat cells in white adipose tissue, binds to and activates its cognate receptor, the leptin receptor (LEP-R). Still, smaller quantities have been detected in other body tissues, including the brown adipose tissue (BAT), placenta, fetal tissue, stomach, muscles, bone marrow, teeth, and brain. Leptin circulates in the blood in both free and protein-bound forms, where the free form of leptin is the biologically active form. Leptin can enter the central nervous system (CNS) (in the area of the choroid plexus) by receptor-mediated transport. Leptin secretion is higher in subcutaneous than in visceral adipose tissue.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LEP, OB, Obese protein, Obesity factor, OBS |